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GeneArt® CRISPR Nuclease Vector with OFP Reporter Kit
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产品名称: GeneArt® CRISPR Nuclease Vector with OFP Reporter Kit
产品型号: A21174





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产品描述

货号: A21174

 

规格

 

Promoter: CMV, U6
Reporter: OFP
Key Function: Episomal Expression, Genome Engineering, Gene Editing, Stable Cell Line Development
Product Size: 10 reactions
Cloning Method: Restriction Enzyme ⁄ MCS
Delivery Method: Transfection
Protein Tag or Fusion: OFP fusion

 

内容及储存

 

Contains:
• CRISPR OFP Nuclease Vector, linearized
• 10X Oligonucleotide Annealing Buffer
• DNase/RNase-free Water
• 5X Ligation Buffer
• T4 DNA Ligase
• U6 Forward Sequencing Primer
• Control Double-stranded Oligonucleotide

Store in freezer (-5 to -30°C).

 

描述

 

The GeneArt® CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells. The linearized GeneArt® CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease.

The GeneArt® CRISPR Nuclease Vector system offers:

• Easy-to-design genome engineering system
• Affordable, ready-to-use cloning vectors
• Enrichment for hard to transfect or difficult to modify cell lines

All-in-one Vector System for CRISPR-based Genome Editing
The GeneArt® CRISPR Nuclease Vector kit offers a simple, ready-to-use, all-in-one expression vector system consisting of both a Cas9 nuclease expression cassette and a guide RNA (gRNA) cloning cassette for rapid and efficient cloning of DNA that encodes target-specific CRISPR RNA (crRNA). This system allows you to edit and engineer a genomic locus of choice in a sequence-specific manner from a single plasmid. After relevant targets have been identified with fast and easy-to-use GeneArt® CRISPR vectors, the biologically relevant mutations can be precisely created with GeneArt® Precision TALs, with high specificity and low off-target effects.

 

 

 

 

 

 

 

 

 

 

 
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电话:010-56429247
手机:13488645491